abstract：:Lactosylated poly-L-lysine is a nonviral vector that transfers genes into airway epithelial cells, including those from individuals with cystic fibrosis (CF). Substitution of 40% of the epsilon-amino groups of poly-L-lysine with lactosyl residues not only provided a ligand for receptor-mediated endocytosis, but also r...

journal_title：American journal of respiratory cell and molecular biology

authors： Kollen WJ,Schembri FM,Gerwig GJ,Vliegenthart JF,Glick MC,Scanlin TF

更新日期：1999-05-01 00:00:00

abstract：:Mucus hypersecretion is characteristic of chronic airway diseases. However, regulatory mechanisms are poorly understood. Human airway epithelial cells grown on permeable supports at the air-liquid interface (ALI) develop a mucociliary morphology resembling that found in vivo. Such cultures provide a model for studying...

journal_title：American journal of respiratory cell and molecular biology

authors： Bernacki SH,Nelson AL,Abdullah L,Sheehan JK,Harris A,Davis CW,Randell SH

更新日期：1999-04-01 00:00:00

abstract：:Human tracheal gland serous (HTGS) cells are now considered one principal pulmonary target for the gene therapy of cystic fibrosis (CF). We developed a CF tracheal gland serous cell line, CF-KM4, obtained by the transformation of primary cultures of CF tracheal gland serous cells homozygous for the DeltaF508 mutation ...

journal_title：American journal of respiratory cell and molecular biology

authors： Kammouni W,Moreau B,Becq F,Saleh A,Pavirani A,Figarella C,Merten MD

更新日期：1999-04-01 00:00:00

abstract：:In rat models of Gram-negative pneumonia, pulmonary emigration of neutrophils (polymorphonuclear leukocytes [PMNs]) is blocked when rats are made endotoxemic by an intravenous administration of endotoxin (lipopolysaccharide [LPS]). To test whether dysfunctional PMN migratory responses in the endotoxemic rat are specif...

journal_title：American journal of respiratory cell and molecular biology

authors： Wagner JG,Driscoll KE,Roth RA

更新日期：1999-04-01 00:00:00

abstract：:Keratinocyte growth factor (KGF) or fibroblast growth factor (FGF)-7, a peptide produced by stromal cells and in particular by lung mesenchyme, has recently been shown to influence early lung morphogenesis and to be a mitogen for fetal and adult alveolar type II cells. Although contradictory findings have been reporte...

journal_title：American journal of respiratory cell and molecular biology

authors： Chelly N,Mouhieddine-Gueddiche OB,Barlier-Mur AM,Chailley-Heu B,Bourbon JR

更新日期：1999-03-01 00:00:00

abstract：:Infants born to heroin- and cocaine-addicted mothers have been reported to have a lower incidence of respiratory distress syndrome (RDS) compared with nonaddicted infants. However, it is not known whether these are direct drug-mediated effects or secondary phenomena. We therefore investigated the effect of opioids and...

journal_title：American journal of respiratory cell and molecular biology

authors： Gewolb IH,O'Brien J,Slavin RE

更新日期：1999-03-01 00:00:00

abstract：:We have previously demonstrated that the asthma-associated proinflammatory eicosanoid leukotriene D4 (LTD4) is co-mitogenic with insulin-like growth factors (IGFs) in airway smooth-muscle (ASM) cells in vitro. This synergistic effect of LTD4 and IGF on ASM cell growth involves proteolysis of ASM-produced IGF binding p...

journal_title：American journal of respiratory cell and molecular biology

authors： Rajah R,Nachajon RV,Collins MH,Hakonarson H,Grunstein MM,Cohen P

更新日期：1999-02-01 00:00:00

abstract：:Chronic obstructive pulmonary disease (COPD) has been associated with heterozygosity for the Z and S alleles of the alpha1-antitrypsin gene in some studies, but these observations have not been confirmed by others. Cigarette smoking is the major risk factor for COPD and may have been a confounding factor in many of th...

journal_title：American journal of respiratory cell and molecular biology

authors： Sandford AJ,Weir TD,Spinelli JJ,Paré PD

更新日期：1999-02-01 00:00:00

abstract：:Although mast cell secretion has been intensively studied because of its pivotal role in allergic reactions and its advantages as a physiologic model, the molecular composition of the secretory machine is virtually unknown. In view of the guanine-nucleotide dependency of mast cell exocytosis and the participation of R...

journal_title：American journal of respiratory cell and molecular biology

authors： Tuvim MJ,Adachi R,Chocano JF,Moore RH,Lampert RM,Zera E,Romero E,Knoll BJ,Dickey BF

更新日期：1999-01-01 00:00:00

abstract：:5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE) is a metabolite of arachidonic acid formed by the oxidation of 5-hydroxy-6,8,11, 14-eicosatetraenoic acid by a highly specific dehydrogenase. 5-oxo-ETE is a chemoattractant for both neutrophils and eosinophils. Although it is not as effective as leukotriene B4 (LTB4) a...

journal_title：American journal of respiratory cell and molecular biology

authors： Powell WS,Gravel S,Halwani F

更新日期：1999-01-01 00:00:00

abstract：:The oleic acid (OA) model of acute lung injury in rats is characterized by a massive and rapid influx of polymorphonuclear neutrophils (PMN) within 1 h, with a peak inflammatory response at 4 h and resolution by 72 h. We hypothesized that PMN apoptosis is involved in the resolution of OA-induced acute lung injury. To ...

journal_title：American journal of respiratory cell and molecular biology

authors： Hussain N,Wu F,Zhu L,Thrall RS,Kresch MJ

更新日期：1998-12-01 00:00:00

abstract：:We studied the effect of the nitric oxide synthase (NOS) inhibitor asymmetric dimethyl arginine (ADMA) and the inactive enantiomer N G-methyl-D-arginine (D-NMMA) on Pseudomonas aeruginosa infection of the respiratory mucosa in nasal turbinate organ cultures. We also investigated the effect of P. aeruginosa culture fil...

journal_title：American journal of respiratory cell and molecular biology

authors： Dowling RB,Newton R,Robichaud A,Cole PJ,Barnes PJ,Wilson R

更新日期：1998-12-01 00:00:00

abstract：:Myosin light chain phosphorylation results in cellular contraction and is a critical component of agonist-mediated endothelial cell (EC) junctional gap formation and permeability. We have shown that this reaction is catalyzed by a novel high molecular-weight Ca2+/calmodulin-dependent nonmuscle myosin light chain kinas...

journal_title：American journal of respiratory cell and molecular biology

authors： Verin AD,Lazar V,Torry RJ,Labarrere CA,Patterson CE,Garcia JG

更新日期：1998-11-01 00:00:00

abstract：:Alveolar macrophages (AMs) play an important role in the regulation of the local immune reactivity in the lung. It was previously shown that exposure of rats to mild inescapable electrical footshock stress (20 min, 4 shocks/min, 5 s/shock, 0.8 mAmp) leads to apparent changes in the activity of AMs upon stimulation, re...

journal_title：American journal of respiratory cell and molecular biology

authors： Broug-Holub E,Persoons JH,Schornagel K,Mastbergen SC,Kraal G

更新日期：1998-11-01 00:00:00

abstract：:Lung injury is a frequent consequence of oxygen (O2) therapy administered to newborns and adults with respiratory distress. Acute exposure to hyperoxia results in a well-described pathophysiologic response in the lungs. Because inflammation is an important component of pulmonary O2 toxicity, we have an interest in ide...

journal_title：American journal of respiratory cell and molecular biology

authors： Piedboeuf B,Gamache M,Frenette J,Horowitz S,Baldwin HS,Petrov P

更新日期：1998-10-01 00:00:00

abstract：:Clara cell secretory protein (CCSP), or CC10, is an inhibitor of secretory phospholipase A2 which may be produced by phagocytic cells and by a variety of other cells in the airway. Tumor necrosis factor-alpha (TNF-alpha) is capable of activating phospholipases and inducing the expression of a variety of genes in the a...

journal_title：American journal of respiratory cell and molecular biology

authors： Yao XL,Levine SJ,Cowan MJ,Logun C,Shelhamer JH

更新日期：1998-10-01 00:00:00

abstract：:Previously it was reported that hyperoxia induced death of the human lung adenocarcinoma cell line (A549 cells) by necrosis, not by apoptosis. This study examined proliferation and death of untransformed human small airway epithelial (SAE) cells in normoxia or hyperoxia in comparison with A549 cells. We tested the hyp...

journal_title：American journal of respiratory cell and molecular biology

authors： Jyonouchi H,Sun S,Abiru T,Chareancholvanich S,Ingbar DH

更新日期：1998-09-01 00:00:00

abstract：:The capacity of Mycobacterium tuberculosis (MTB) to induce production of chemokines with known chemotactic activity for monocytes and lymphocytes, the cellular building blocks of granulomas, was investigated. These chemokines included regulated upon activation, normal T cell expressed and secreted (RANTES), monocyte c...

journal_title：American journal of respiratory cell and molecular biology

authors： Sadek MI,Sada E,Toossi Z,Schwander SK,Rich EA

更新日期：1998-09-01 00:00:00

abstract：:The surfactant-associated proteins SP-A and SP-D are members of a family of collagenous host defense lectins, designated collectins. There is increasing evidence that these pulmonary epithelial-derived proteins are important components of the innate immune response to microbial challenge, and that they participate in ...

journal_title：American journal of respiratory cell and molecular biology

authors： Crouch EC

更新日期：1998-08-01 00:00:00

abstract：:Congenital diaphragmatic hernia (CDH) is associated with high neonatal mortality from lung hypoplasia and persistent pulmonary hypertension. Pulmonary neuroendocrine cells (PNEC) produce calcitonin gene-related peptide (CGRP), a potent vasodilator. We previously reported altered distribution of CGRP-positive PNEC in f...

journal_title：American journal of respiratory cell and molecular biology

authors： IJsselstijn H,Hung N,de Jongste JC,Tibboel D,Cutz E

更新日期：1998-08-01 00:00:00

abstract：:Higher numbers of eosinophil/basophil colony-forming units (Eo/B CFU) are observed in blood of atopic individuals, and can be enhanced in atopic asthmatics by allergen-inhalation challenge. It is known that mature basophils and eosinophils synthesize cytokines relevant to allergic inflammation. To investigate the pote...

journal_title：American journal of respiratory cell and molecular biology

authors： Gauvreau GM,O'Byrne PM,Moqbel R,Velazquez J,Watson RM,Howie KJ,Denburg JA

更新日期：1998-07-01 00:00:00

abstract：:T1alpha is a protein of unknown function that is expressed at the plasma membrane in epithelia involved in fluid transport, including type I alveolar epithelial cells, choroid plexus, and ciliary epithelium. The purpose of this study was to test the hypothesis that T1alpha functions as a water channel or a regulator o...

journal_title：American journal of respiratory cell and molecular biology

authors： Ma T,Yang B,Matthay MA,Verkman AS

更新日期：1998-07-01 00:00:00

abstract：:Increases in inflammatory-cell progenitors have been demonstrated in the bone marrow (BM) after inhalation of Ascaris suum in dogs at the time of allergen-induced airway hyperresponsiveness (AHR). The aim of this study was to evaluate the effect of allergen challenge on trafficking of inflammatory cells and their prog...

journal_title：American journal of respiratory cell and molecular biology

authors： Wood LJ,Inman MD,Denburg JA,O'Byrne PM

更新日期：1998-06-01 00:00:00

abstract：:Nitric oxide (NO) is produced in lung epithelial cells by nitric oxide synthases (NOSs), which can enhance inflammation and edema formation. The inducible NOS (iNOS, type II NOS) has been shown to be increased in lung disorders such as asthma. Therapy for asthma includes antiinflammatory agents such as corticosteroids...

journal_title：American journal of respiratory cell and molecular biology

authors： Robbins RA,Jinkins PA,Bryan TW,Prado SC,Milligan SA

更新日期：1998-06-01 00:00:00

abstract：:We investigated mechanically induced cell-to-cell Ca2+ signaling in a preparation of rabbit tracheal epithelium close to its in vivo condition. We used confocal microscopy to analyze changes in intracellular free calcium concentration ([Ca2+]i) in intact ciliated tracheal mucosal explants loaded with the Ca2+-indicato...

journal_title：American journal of respiratory cell and molecular biology

authors： Felix JA,Chaban VV,Woodruff ML,Dirksen ER

更新日期：1998-05-01 00:00:00

abstract：:Previous studies have shown that a single exposure of animals to ozone (O3) can induce protection or adaptation to the acute injurious effects of a subsequent O3 challenge. Although a number of mechanisms have been proposed to account for this response, none appear to be fully explanatory. We examined the role interle...

journal_title：American journal of respiratory cell and molecular biology

authors： McKinney WJ,Jaskot RH,Richards JH,Costa DL,Dreher KL

更新日期：1998-05-01 00:00:00

abstract：:Emphasis has recently been placed on the roles of chemotactic cytokines called chemokines to explain the accumulation of inflammatory cells in the lung that may precede or accompany pulmonary fibrosis in interstitial lung diseases. We hypothesized that RANTES, a member of the C-C chemokines, is one such chemokine. Bro...

journal_title：American journal of respiratory cell and molecular biology

authors： Kodama N,Yamaguchi E,Hizawa N,Furuya K,Kojima J,Oguri M,Takahashi T,Kawakami Y

更新日期：1998-04-01 00:00:00

abstract：:In this study, we investigated the effect of the novel retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalene carboxylic acid (AHPN/CD437) on the growth of human lung carcinoma cell lines. AHPN inhibits the proliferation of all cell lines tested, irrespective of the lung tumor type, in a concentration- and time-de...

journal_title：American journal of respiratory cell and molecular biology

authors： Adachi H,Preston G,Harvat B,Dawson MI,Jetten AM

更新日期：1998-03-01 00:00:00

abstract：:Inhaled nitric oxide (NO) is an important new therapeutic agent used to treat pulmonary arterial hypertension in a variety of disease states. However, the effects of NO on cells in the lung are uncertain. Previously, we have shown that NO gas depresses neutrophil oxidative cell function and increases neutrophil cell d...

journal_title：American journal of respiratory cell and molecular biology

authors： Fortenberry JD,Owens ML,Brown MR,Atkinson D,Brown LA

更新日期：1998-03-01 00:00:00

abstract：:Heme oxygenase-1 (HO-1) is a stress-response protein, the expression of which is transcriptionally regulated by agents that cause oxidative stress. We have previously shown that lipopolysaccharide (LPS)-induced HO-1 gene transcription in RAW 264.7 macrophage cells is mediated by a distal enhancer called SX2, located 4...

journal_title：American journal of respiratory cell and molecular biology

authors： Camhi SL,Alam J,Wiegand GW,Chin BY,Choi AM

更新日期：1998-02-01 00:00:00

abstract：:We examined the ability of the human surfactant protein B (SP-B) promoter to confer cell specificity of transgene expression in an adenoviral vector. Using similar replication-deficient adenoviruses (rAd), we compared lacZ reporter gene expression driven by the human SP-B promoter (rAd.SPBlacZ) with the ubiquitously e...

journal_title：American journal of respiratory cell and molecular biology

authors： Strayer MS,Guttentag SH,Ballard PL

更新日期：1998-01-01 00:00:00

abstract：:Ozone (O3) is the principal oxidant pollutant in photochemical smog. Repeated exposures to O3 induces inflammation and mucous cell metaplasia in the nasal airways of laboratory animals. Our study was designed to determine the efficacy of a topical anti-inflammatory corticosteroid in preventing O3-induced rhinitis and ...

journal_title：American journal of respiratory cell and molecular biology

authors： Hotchkiss JA,Hilaski R,Cho H,Regan K,Spencer P,Slack K,Harkema JR

更新日期：1998-01-01 00:00:00

abstract：:A murine model was used to assess the role of cytokines in initiating protective T-cell-mediated immunity in the lung. A pulmonary infection was initiated by intratracheal inoculation of Cryptococcus neoformans (Cne). Previously, we had established that Cne lung clearance was mouse-strain-specific: C.B-17 mice were re...

journal_title：American journal of respiratory cell and molecular biology

authors： Hoag KA,Lipscomb MF,Izzo AA,Street NE

更新日期：1997-12-01 00:00:00

abstract：:Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Reid CJ,Gould S,Harris A

更新日期：1997-11-01 00:00:00

abstract：:Extracellular signal-regulated kinases (ERKs) phosphorylate and regulate cytoskeletal components of contractile cells and have been implicated in integrin-mediated adhesion. In this study, we examined the contributions of adherence, cell flattening, and cytoskeletal reorganization to adhesion-induced ERK activation in...

journal_title：American journal of respiratory cell and molecular biology

authors： Heuertz RM,Hamann KJ,Hershenson MB,Leff AR

更新日期：1997-10-01 00:00:00

abstract：:At birth, the mammalian lung is still immature. The alveoli are not yet formed and the interairspace walls contain two capillary layers which are separated by an interstitial core. After alveolarization (first 2 postnatal weeks in rats) the alveolar septa mature: their capillary layers merge, the amount of connective ...

journal_title：American journal of respiratory cell and molecular biology

authors： Schittny JC,Paulsson M,Vallan C,Burri PH,Kedei N,Aeschlimann D

更新日期：1997-09-01 00:00:00

abstract：:Tumor necrosis factor alpha (TNF-alpha), a major product of alveolar macrophages (AM), has been implicated in many pulmonary diseases. Histamine, a mediator important in pulmonary inflammation, has been demonstrated to regulate the production of TNF-alpha by monocytes. In this study, we show that human AM and monocyte...

journal_title：American journal of respiratory cell and molecular biology

authors： Rowe J,Finlay-Jones JJ,Nicholas TE,Bowden J,Morton S,Hart PH

更新日期：1997-08-01 00:00:00

abstract：:The lung epithelium has recently been identified as a novel site of fibrinogen (FBG) biosynthesis. A coordinated upregulation of A alpha, B beta, and gamma chain FBG gene transcription occurs upon stimulation of A549 lung epithelial cells with dexamethasone (DEX) and the proinflammatory mediator interleukin-6 (IL-6). ...

journal_title：American journal of respiratory cell and molecular biology

authors： Guadiz G,Sporn LA,Goss RA,Lawrence SO,Marder VJ,Simpson-Haidaris PJ

更新日期：1997-07-01 00:00:00

abstract：:Extensive tissue remodeling occurs in survivors of acute lung injury, leading to nearly normal histology and physiology in the majority of individuals, whereas others suffer significant impairment due to the development of pulmonary fibrosis. Alveolar epithelial cells play a central role in the repair process. They ar...

journal_title：American journal of respiratory cell and molecular biology

authors： Hasegawa T,Sorensen L,Dohi M,Rao NV,Hoidal JR,Marshall BC

更新日期：1997-06-01 00:00:00

abstract：:Alveolar macrophages (AM) and interstitial macrophages (IM) from rat lungs were characterized with respect to morphology, phagocytosis, adhesion properties, and phenotype. AM were recovered by lung lavage and IM by treatment of the lung tissue with DNAse and collagenase. The AM were enzyme treated in the same way as t...

journal_title：American journal of respiratory cell and molecular biology

authors： Johansson A,Lundborg M,Sköld CM,Lundahl J,Tornling G,Eklund A,Camner P

更新日期：1997-05-01 00:00:00